Application of RAPD Methods in Meat for Beef Breed Identification

Choy, Y. H.; Oh, S. J.; Kang, J. O.

doi:2001.14.12.1655

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2001;14(12): 1655-1658.
https://doi.org/10.5713/ajas.2001.1655 Published online December 1, 2001.

Application of RAPD Methods in Meat for Beef Breed Identification

Y. H. Choy, S. J. Oh, J. O. Kang

Abstract

Bovine genome samples were collected from meat of three different beef breeds (Hanwoo, Holstein and imported beef breed) that are commercially merchandized in Korean beef market. Operon B (OPB)-kits were used as random primers (3, 7, 10, 11, 12, 14) in random amplified polymorphic DNA (RAPD) method on whole genome. Each primer provided characteristic bands that were highly polymorphic. Each single primer could provide relatively efficient polymorphic band patterns among breeds. However, use of two or more primers in combination is recommended to improve resolution of experiments with higher molecular weight bands of DNA. In our experiments, OPB-11 resolved well between beef cattle breeds and Holstein. And OPB-7, 12 and 14 could be combined with OPB-11 to identify Hanwoo beef from the other two kinds of beef.

Keywords: Hanwoo; Holstein; Random Amplified Polymorphic DNA