Stable transformation of cultured chicken cells

Han, J. Y.; Shin, Y. S.; Shoffner, R. N.; Guise, K. S.

doi:1993.6.4.581

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 1993;6(4): 581-589.
https://doi.org/10.5713/ajas.1993.581 Published online December 1, 1993.

Stable transformation of cultured chicken cells

J. Y. Han, Y. S. Shin, R. N. Shoffner, K. S. Guise

Abstract

A plasmid vector, RSVLTR/棺G2, containing lacZ gene under the control of the RSVLTR promoter were transfected into chicken embryo fibroblasts by three different transfection methods. Calcium phosphate, lipsome and DEAE-dextran techniques were applied for transfection of chicken cells. A histochemical assay with X-gal was used as a simple method for screening transfected cells. Plasmid RSVLTR/棺G2-was expressed proficiently in the chicken embryo fibroblast. Calcium phosphate-DNA precipitate transfection resulted in the highest efficiency for transient expression of RSVLTR/棺G2. Transfected cells formed colonies on the 9th day of incubation indicating stable transformation of the inserted plasmid.

Keywords: Plasmid Vector; Transfection; Calcium Phosphate; Liposome; DEAE-Dextran; Chicken