The effects of prostaglandins and indomethacin on ovulation in the perfused fowl ovary |
K. Tanaka, T. Higuchi, M. Tawarazumita, F. Hertelendy |
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Abstract |
The effects of prostaglandins (PG) and indomethacin, a PG synthesis inhibitor, on ovulation in the perfused fowl ovary were studied. Laying hens were killed by cutting the jugular vein 18-19 h before expected ovulation of the second follicle of a clutch sequence. The cannulated ovary was dissected free and connected with a recycling perfusion apparatus. Agents to be studied for their effects on ovulation were added to the perfusion fluid. PGF2慣 at 0.1 and 1 mg/l induced ovulation, with a success rate of 25% and 30%, respectively. The same doses of PGF2 were effective at 60% and 63%, respectively. Indomethacin partially blocked gonadotrophin-induced ovulation. It is suggested that PGs may play a supportive role in the process of follicular rupture in the domestic fowl. |
Keywords:
Fowl Ovary; Perfusion; Prostaglandin; Indomethacin |
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