Gene | Full name | Function | GenBank accession number | Primer (5′-3′) | Reference | Length (bp) | qPCR efficiency |
---|---|---|---|---|---|---|---|
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | Carbohydrate metabolism | AF017079 |
F: TCGGAGTGAACGGATTTG R: CCTGGAAGATGGTGATGG |
Martino et al., 2011 | 219 | 97.90 |
ACTB | Beta-actin | Involved in cell motility, structure and integrity | DQ178130 |
F: TCTGGCACCACACCTTCT R: GATCTGGGTCATCTTCTCAC |
Martino et al., 2011 | 114 | 101.20 |
B2M | Beta-2-microglobulin | Ctoskeletal protein involved in cell locomotion | NM 213978 |
F: TTCACACCGCTCCAGTAG R:CCAGATACATAGCAGTTCAGG |
Martino et al., 2011 | 166 | 109.30 |
TBP | TATA binding protein | Transcription initiation from RNA polymerase II promotor | DQ178129 |
F: GATGGACGTTCGGTTTAGG R: AGCAGCACAGTACGAGCAA |
Martino et al., 2011 | 124 | 101.40 |
RPL4 | Ribosomal protein L4 | Structural constituent of ribosome | DQ845176 |
F: AGGAGGCTGTTCTGCTTCTG R: TCCAGGGATGTTTCTGAAGG |
Cinar et al., 2012 | 185 | 97.8 |
PPIA | Peptidylprolyl isomerase A (cyclophilin A) | A member of the peptidylprolyl cis-trans isomerase (PPIase) family | NM_214353 |
F: CACAAACGGTTCCCAGTTTT R: TGTCCACAGTCAGCAATGGT |
Cinar et al., 2012 | 171 | 95.3 |
Sample type | Experimental group | GAPDH | ACTB | B2M | TBP | RPL4 | PPIA |
---|---|---|---|---|---|---|---|
PBMC1 | Non-stimulation | 30.18±1.72 | 20.75±1.00a | 22.24±1.22 | 29.35±1.23 | 25.73±1.83 | 22.28±1.03 |
Poly I:C stimulation | 30.55±1.74 | 22.15±1.29b | 22.07±1.18 | 29.38±1.15 | 25.14±1.62 | 22.33±1.00 | |
All samples of PBMC | 30.42±1.68 | 21.68±1.36 | 22.13±1.16 | 29.37±1.14 | 25.34±0.66 | 22.32±0.98 | |
Whole blood | Non-stimulation | 31.55±1.11 | 22.07±1.46 | 24.27±1.48 | 31.38±1.05 | 28.66±1.67 | 24.56±1.66 |
Poly I: C stimulation | 30.95±1.64 | 22.48±0.85 | 23.53±1.34 | 31.42±1.48 | 28.83±1.97 | 24.10±1.46 | |
All samples of whole blood | 31.15±1.48 | 22.34±1.06 | 23.78±1.39 | 31.41±1.32 | 28.77±1.82 | 24.26±1.50 | |
All samples | 30.79±1.60 | 22.01±1.25 | 22.95±1.52 | 30.39±1.60 | 27.05±2.45 | 23.29±1.59 |