Cytotec Induced Embryotoxicity in Developing Mus musculus

Naeem, Khadija; Ahmad, Naveed; Asmatullah,

doi:2010.23.10.1282

Article

Animal Reproduction and Physiology

Asian-Australasian Journal of Animal Sciences 2010;23(10): 1282-1290.
https://doi.org/10.5713/ajas.2010.90636 Published online August 20, 2010.

Cytotec Induced Embryotoxicity in Developing Mus musculus

Khadija Naeem, Naveed Ahmad, Asmatullah

Abstract

The study was carried out to assess the developmental abnormalities induced by Cytotec in mice during intrauterine life. Pregnant mice were exposed to a single dose of 0, 0.02, 0.04, 0.06, 0.08 and 0.1 g/g BW on day 8 of gestation. Fetuses were recovered on day 18 of gestation. These fetuses were subjected to morphological and morphometric studies. Morphological studies showed abnormalities like anophthalmia, microophthalmia, micromelia and syndactyly. In addition to these, resorptions were also encountered in the higher dose groups. Morphometric analysis showed an overall reduction in body weight, crown rump length, brain and eye circumference, pinna and snout size, length of fore and hind limb and tail size with a significant difference (p<0.001) compared to controls. The outcomes of histological studies revealed some brain defects like hydrocephaly, enlarged third ventricle and undifferentiated ectoneural cells and abnormalities of the heart included right auricle thrombosis and degeneration of trabecular zone.

Keywords: Cytotec; Embryotoxicity; Developmental Anomalies; Mice