Postnatal Expression Pattern of Adipose Type Fatty Acid Binding Protein in Different Adipose Tissues of Porcine

Xu, C. L.; Wang, Y. Z.; Huang, Y. H.; Liu, J. X.; Feng, J.

doi:2007.20.6.811

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2007;20(6): 811-816.
https://doi.org/10.5713/ajas.2007.811 Published online May 2, 2007.

Postnatal Expression Pattern of Adipose Type Fatty Acid Binding Protein in Different Adipose Tissues of Porcine

C. L. Xu, Y. Z. Wang^*, Y. H. Huang, J. X. Liu, J. Feng

Correspondence: Y. Z. Wang,

Abstract

Adipocyte fatty acid-binding protein (A-FABP), which belongs to the FABP family, plays an essential role in long-chain fatty acid uptake and metabolic homeostasis, especially in adipose tissue. The pattern of A-FABP gene mRNA expression in different growth stages and its relation to intramuscular fat (IMF) accretion in pigs was studied. Fifteen female Duroc LandraceYorkshire pigs in five groups of three pigs each, weighing 1, 30, 50, 70 and 90 kg were used to study developmental gene mRNA expression of A-FABP in various adipose tissues by means of semi-quantitative RT-PCR. Results showed that A-FABP mRNA levels in subcutaneous and ventral adipose tissues first increased from 1 to 50 kg, then gradually declined from 50 to 90 kg. Moreover, the rank order of A-FABP mRNA levels determined in three adipose tissues was as follows: subcutaneous adipose>ventral adipose>mesenteric adipose. A-FABP mRNA expression in mesenteric adipose tissue was constant during development. In addition, a positive correlation from 1 to 50 kg BW pigs and a negative correlation from 50 to 90 kg BW between A-FABP mRNA levels in subcutaneous and ventral adipose and IMF content were found.

Keywords: A-FABP; Intramuscular Fat; Gene Expression; Fat Deposition; Pigs