Association between PCR-RFLP Polymorphisms of Five Gene Loci and Milk Traits in Chinese Holstein

Zhang, R. F.; Chen, H.; Lei, C. Z.; Fang, X. T.; Zhang, Y. D.; Hu, S. R.; Su, L. H.

doi:2007.20.2.166

Article

Animal Breeding and Genetics

Asian-Australasian Journal of Animal Sciences 2007;20(2): 166-171.
https://doi.org/10.5713/ajas.2007.166 Published online November 27, 2006.

Association between PCR-RFLP Polymorphisms of Five Gene Loci and Milk Traits in Chinese Holstein

R. F. Zhang, H. Chen^*, C. Z. Lei, X. T. Fang, Y. D. Zhang, S. R. Hu, L. H. Su

Correspondence: H. Chen,

Abstract

The objective of this study was to assess the association of polymorphisms in 觀-cn, ??lg, ??lg 5??flanking region, CSN1S2, and IGFBP-3 genes with milk production traits and mastitis-related traits in Chinese Holstein. Traits analyzed were 305 day standard milk yield, protein percentage, fat percentage, the ratio of fat percentage and protein percentage, pre-somatic cell count, somatic cell count, and somatic cell score, respectively. CSN1S2 locus was uninformative because only one genotype BB was found in Chinese Holstein. Allele frequencies of A and B in IGFBP-3 gene were 0.5738 and 0.4262 in Chinese Holstein population, which was different from reported Qinchuan cattle population. The genotypes of animals at IGFBP-3 locus significantly affected 305 day standard milk yield, protein percentage, and somatic cell score. The ??lg genotypes had a significant effect on protein percentage and the ratio of fat percentage and protein percentage. Polymorphism in ??lg 5??flanking region was associated with 305 day standard milk yield, protein percentage, fat percentage, pre-somatic cell count, and somatic cell count. No significant associations of the polymorphism in ??cn gene were observed for any trait.

Keywords: Chinese Holstein; beta-lg; kappa-cn; IGFBP-3; Milk Traits