Effect of 65 kDa Buffalo Placental Protein on B-Cell Proliferation and Antibody Response

Deepthi, K.; Rajput, Y. S.

doi:2002.15.3.432

Article

Animal Products

Asian-Australasian Journal of Animal Sciences 2002;15(3): 432-437.
https://doi.org/10.5713/ajas.2002.432 Published online March 1, 2005.

Effect of 65 kDa Buffalo Placental Protein on B-Cell Proliferation and Antibody Response

K. Deepthi, Y. S. Rajput

Abstract

Immunosuppressive potential of 65 kDa buffalo placental protein (bPP65) on B-cell proliferation in vitro and antibody response in vivo was evaluated. B-cell proliferation was estimated by measuring incorporation of tritiated thymidine in buffalo lymphocytes while primary antibody responses against phytohaemagglutinin (PHA) or keyhole limpet haemocyanin (KLH) were evaluated in mice. bPP65 suppressed proliferation of lipopolysaccharide (a B-cell specific mitogen)-stimulated buffalo lymphocytes in vitro indicating suppression of B-cells. This suppression was dose dependent over the protein concentration range 25-100 g/ml. Primary antibody responses in mice against PHA and KLH in presence of bPP65 were lower as compared to in its absence but these were not statistically significant. Amino acid composition data of bPP65 and BSA suggested that bPP65 is different from BSA.

Keywords: Placental Protein; Immunosuppressive Protein; Antibody Response; B-Cell Proliferation