Cloning and Expression of β1-Adrenergic Receptor Genes in Adipose Tissues from Korean Native Cattle (Hanwoo)

Ha, S. H.; Chung, M. I.; Baik, M. G.; Choi, Y. J.

doi:2001.14.1.13

Article

Animal Reproduction and Physiology

Asian-Australasian Journal of Animal Sciences 2001;14(1): 13-16.
https://doi.org/10.5713/ajas.2001.13 Published online January 1, 2001.

Cloning and Expression of β1-Adrenergic Receptor Genes in Adipose Tissues from Korean Native Cattle (Hanwoo)

S. H. Ha, M. I. Chung, M. G. Baik, Y. J. Choi

Abstract

Bovine 횩1-adrenergic receptor (AR) cDNA was cloned using degenerative primers. Bovine 횩1-AR coded for 467 amino acids and the comparison of the deduced amino acid sequence with that of sheep showed 93.4% identity. Northern blot analysis indicated that transcript size for the bovine 횩1-AR was 3.6 kb in the adipose tissue. The expression level of three 횩-ARs (1, 2, and 3) in bovine abdominal, subcutaneous, and perirenal adipose tissues were examined using reverse transcription-polymerase chain reaction (RT-PCR), and the levels of 횩1- and 횩3-AR mRNA were found to be lower in the subcutaneous adipose tissue than in the abdominal and perirenal adipose tissues. These results suggest that the expression of 횩-ARs mRNA are differentially regulated among the adipose tissues.

Keywords: 횩1-Adrenergic Receptor cDNA; Cloning; Expression; Adipose Tissue