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Sequence analysis and comparison of bovine as1-casein genomic DNA |
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C. S. Lin, M. C. Huang, K. B. Choo, Y. H. Tseng |
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| Abstract |
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A phage clone containing the partial 慣S1-casein gene was isolated from a bovine genomic library by using mixed probes of ovine 慣S1-, 棺- and k-casein cDNAs. Restriction enzyme mapping analysis for 14.6 kb revealed that the map was in conflict with the report of Meade et al. (1990), especially in the 3`-end fragment. Sequence analysis of 12.6 kb revealed a high AT/GC ratio (1.64); we have identified eight exon sequences according to the bovine 慣S1-casein cDNA sequence. The same exon/intron splice junction sequence was observed between these exons. We suggest that the bovine 慣S1-casein gene night contain a minimum of 18 exons and the full length is approximately 18-19 kb. |
| Keywords:
Bovine 慣S1-Casein Gene; Genomic DNA; Restriction Map; Exon/Intron |
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