Anim Biosci > Volume 29(11); 2016 > Article |
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AUTHOR CONTRIBUTIONS
HM collected the blood samples, carried out the extraction of genomic DNA, PCR, and DNA sequencing, performed the genetic data analysis and participated in the writing of the manuscript. CPJ collected the blood samples from Iran and helped the extraction of genomic DNA. MAY conceived of the study, participated in its coordination, performed the statistical analysis and participated in the writing of the manuscript. SW participated in writing of the manuscript and interpretation of results. All authors read and approved the final manuscript.
Haplotype | Accession no. | Reference |
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TRIRE1 | KT596789 | This study |
TRE2–TRE4 | KT596790–KT596792 | This study |
IRE5 | KT596793 | This study |
IRA | KT596794 | This study |
IRE6–IRE18 | KT596795–KT596807 | This study |
Liu_A1 | AB114069 | Liu et al., 2006 |
Liu_B1 | AB007744 | Liu et al., 2006 |
Liu_C1 | AB114070 | Liu et al., 2006 |
Liu_D1 | AY588636 | Liu et al., 2006 |
Liu_E1 | AB114076 | Liu et al., 2006 |
Liu_F1 | AF512285 | Liu et al., 2006 |
Liu_G1 | AF512288 | Liu et al., 2006 |
Liu_H1 | D82904 | Liu et al., 2006 |
Liu_I1 | AB009434 | Liu et al., 2006 |
RJF1 | AB007720 | Miyake, 1997 |
RJF2 | AP003322 | Nishibori et al., 2005 |
Clades | Turkey (TR)* | Iran (IR)* |
Total n = 222 |
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TRD n = 31 |
TRG n = 23 |
IRWM n = 23 |
IRBM n = 30 |
IRNN n = 22 |
IRCB n = 23 |
IRNH n = 23 |
IRLR n = 24 |
IRWA n = 23 |
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TRIRE1 | 26 | 21 | 19 | 23 | 22 | 20 | 19 | 17 | 14 | 181 |
TRE2 | 5 | 5 | ||||||||
TRE3 | 1 | 1 | ||||||||
TRE4 | 1 | 1 | ||||||||
IRE5 | 3 | 3 | 1 | 7 | ||||||
IRA | 1 | 1 | 1 | 3 | ||||||
IRE6 | 1 | 1 | ||||||||
IRE7 | 3 | 3 | ||||||||
IRE8 | 2 | 1 | 3 | |||||||
IRE9 | 1 | 1 | ||||||||
IRE10 | 1 | 1 | ||||||||
IRE11 | 4 | 4 | ||||||||
IRE12 | 1 | 1 | ||||||||
IRE13 | 1 | 1 | ||||||||
IRE14 | 1 | 1 | ||||||||
IRE15 | 5 | 5 | ||||||||
IRE16 | 1 | 1 | ||||||||
IRE17 | 1 | 1 | ||||||||
IRE18 | 1 | 1 |
Breeds | n | S | h | Hd | π | k | D |
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Denizli (TRD) | 31 | 1 | 2 | 0.279 | 0.0006 | 0.279 | 0.180 |
Gerze (TRG) | 23 | 3 | 3 | 0.170 | 0.0007 | 0.339 | −1.483 |
Total | 54 | 3 | 4 | 0.237 | 0.0007 | 0.311 | −1.071 |
White Marandi (IRWM) | 23 | 10 | 3 | 0.312 | 0.0022 | 1.019 | −2.102* |
Black Marandi (IRBM) | 30 | 3 | 4 | 0.405 | 0.0013 | 0.637 | −0.374 |
Naked Neck (IRNN) | 22 | 0 | 1 | 0.000 | - | - | - |
Common Breed (IRCB) | 23 | 11 | 3 | 0.245 | 0.0022 | 1.027 | −2.092* |
New Hampshire (IRNH) | 23 | 11 | 5 | 0.324 | 0.0027 | 1.264 | −1.797 |
Lari (IRLR) | 24 | 5 | 5 | 0.485 | 0.0019 | 0.876 | −1.000 |
West Azerbaijan (IRWA) | 23 | 11 | 6 | 0.601 | 0.0037 | 1.699 | −1.470 |
Total | 168 | 22 | 17 | 0.361 | 0.0021 | 0.958 | −2.078* |
Total | 222 | 24 | 19 | 0.334 | 0.0017 | 0.802 | −2.178** |
Level of analysis | Variance components (% variation) | p-value | |||
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Within | Among | Total | FST | ||
All nine populations | 0.400 (95.1) | 0.021( 4.9) | 0.421 | 0.049*** | 0.000 |
Two Turkish populations | 0.171 (96.5) | 0.006 (3.5) | 0.177 | 0.035 | 0.083 |
Seven Iranian populations | 0.474 (96.1) | 0.019 ( 3.9) | 0.493 | 0.039 | 0.005 |
Among countries | 0.400 (94.4) | 0.024 ( 5.6) | 0.424 | 0.056*** | 0.000 |